Journal: Analytical Chemistry

Article Title: Optimized Light-Directed Synthesis of Aptamer Microarrays

doi: 10.1021/ac400746j

Figure Lengend Snippet: Fluorescent images of sections of microarrays synthesized on different substrates. (A) Corning UltraGAPS, (B) Schott Epoxy ring-opened, (C) Schott A+, (D) Schott Glass D hydroxyl-functionalized with N -(3-triethoxysilylpropyl)-4-hydroxybutryramide. Green features are hybridization signals from Cy3-labeled sequences. Red features are from Cy5-labeled biotin binding to the streptavidin–aptamer pairs. A scheme identifying the sequences corresponding to the spots can be found in the . The arrays were synthesized with light exposures of 11 J/cm 2 and with 32 × 32 μm features (4 DMD mirrors) separated by gaps of 48 μm. All images were acquired with the same scanner settings.

Article Snippet: − To evaluate the effect of surface chemistry on both aptamer binding and hybridization, we synthesized microarrays containing a variety of hybridization probes and aptamers on four different types of functionalized glass substrates: Corning UltraGAPS (Gamma Amino Propyl Silane), Schott Nexterion Slide A+ (GAPS; 1.0 ± 0.3 × 10 12 molecules/cm 2 ), Schott Nexterion ring-opened Slide E (epoxysilane, 5.6 ± 0.3 × 10 12 molecules/cm 2 ).

Techniques: Synthesized, Hybridization, Labeling, Binding Assay

Journal: Analytical Chemistry

Article Title: Optimized Light-Directed Synthesis of Aptamer Microarrays

doi: 10.1021/ac400746j

Figure Lengend Snippet: St-2-1 Binding Data for Microarrays Synthesized on Different Substrates

Article Snippet: − To evaluate the effect of surface chemistry on both aptamer binding and hybridization, we synthesized microarrays containing a variety of hybridization probes and aptamers on four different types of functionalized glass substrates: Corning UltraGAPS (Gamma Amino Propyl Silane), Schott Nexterion Slide A+ (GAPS; 1.0 ± 0.3 × 10 12 molecules/cm 2 ), Schott Nexterion ring-opened Slide E (epoxysilane, 5.6 ± 0.3 × 10 12 molecules/cm 2 ).

Techniques: Binding Assay, Synthesized